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affinity assay mmp14  (R&D Systems)


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    Structured Review

    R&D Systems affinity assay mmp14
    Affinity Assay Mmp14, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mmp14/Recombinant+Human+MMP-14%2FMT1-MMP+Protein%2C+CF/pm41073424-261-4-7
    Average 93 stars, based on 17 article reviews
    affinity assay mmp14 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: BAY-9835: Discovery of the First Orally Bioavailable ADAMTS7 Inhibitor
    Article Snippet: .. MMP14, ADAM8 and ADAM10 enzymatic assays: Recombinant MMP14 (R&D Systems # 918-MP-010) was diluted in the reaction buffer (50 mM TRIS pH 7,5; 150 mM NaCl; 10 mM CaCl2; 0,05% Brij; 0,1% SmartBlock (Candor Bioscience #113125)) to a concentration of approximately 2 nM and 25 μl was transferred into each single well of a 384-well white microtiter plate (Greiner Bio One 781075). ..

    Article Title: Low protease activity in B cell follicles promotes retention of intact antigens after immunization
    Article Snippet: .. This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10, R&D Systems), ADAM10 (946-AD-020, R&D Systems), Cathepsin D (1029-AS-010, R&D Systems), Cathepsin S (50769-M08H, Sino Biologicals), or Cathepsin L (1515-CY-010, R&D Systems). ..

    Article Title: XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies
    Article Snippet: Primary human peripheral blood mononuclear cells (PBMC; BioIVT) were activated with PMA/Ionomycin (BioLegend, catalog no. 423301) for 4 days at 37°C in RPMI (Gibco, catalog no. 21870–084) with 10% (v/v) FBS (Gibco, catalog no. A38400–01), 1X gentamycin (Gibco, catalog no. 15750–060), 10 mg/L β-mercapto-ethanol (Gibco, catalog no. 21985–023), 1X HEPES (Gibco, catalog no. 15630–080), 1X penicillin/streptomycin (Gibco, catalog no. 15070–063), 1X sodium pyruvate (Gibco, catalog no. 11360–070), 1X MEM (Gibco, catalog no. 11140–050) and 1X l -glutamine (Gibco, catalog no. 25030–081). .. Activated PBMCs were incubated with XTX301, and proteolytically activated XTX301 using recombinant MMP14 (R&D systems, catalog no. 918-MPN) or rh-IL12 (R&D systems, catalog no. 219-IL-025/CF) at 37°C for 30 minutes. ..

    Article Title: Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens
    Article Snippet: .. This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10, R&D Systems), ADAM10 (946-AD-020, R&D Systems), Cathepsin D (1029-AS-010, R&D Systems), Cathepsin S (50769-M08H, Sinobiologicals), or Cathepsin L (1515-CY-010, R&D Systems). ..

    Article Title: XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies
    Article Snippet: The data analysis was performed using MSD Discovery Workbench software. .. 384 IFN- assay 385 XTX301 and mXTX301 were proteolytically activated using recombinant MMP14 (R&D systems, Catalog 386 no. 918-MPN). .. Murine splenocytes were isolated via mechanical dissociation and pre-activated with 387 PMA/Ionomycin (BioLegend, Catalog #423301) for 2 days at 37°C before incubating with varying doses 388 of rm IL-12 (R&D Systems, Catalog #419-ML/CF), murine surrogate mXTX301 or proteolytically activated 389 mXTX301 for 24 hours.

    Article Title: XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies
    Article Snippet: The data analysis was performed using MSD Discovery Workbench software. .. XTX301 and mXTX301 were proteolytically activated using recombinant MMP14 (R&D systems, catalog no. 918-MPN). .. XTX301 and mXTX301 were proteolytically activated using recombinant MMP14 (R&D systems, catalog no. 918-MPN).

    Concentration Assay:

    Article Title: BAY-9835: Discovery of the First Orally Bioavailable ADAMTS7 Inhibitor
    Article Snippet: .. MMP14, ADAM8 and ADAM10 enzymatic assays: Recombinant MMP14 (R&D Systems # 918-MP-010) was diluted in the reaction buffer (50 mM TRIS pH 7,5; 150 mM NaCl; 10 mM CaCl2; 0,05% Brij; 0,1% SmartBlock (Candor Bioscience #113125)) to a concentration of approximately 2 nM and 25 μl was transferred into each single well of a 384-well white microtiter plate (Greiner Bio One 781075). ..

    Incubation:

    Article Title: XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies
    Article Snippet: Primary human peripheral blood mononuclear cells (PBMC; BioIVT) were activated with PMA/Ionomycin (BioLegend, catalog no. 423301) for 4 days at 37°C in RPMI (Gibco, catalog no. 21870–084) with 10% (v/v) FBS (Gibco, catalog no. A38400–01), 1X gentamycin (Gibco, catalog no. 15750–060), 10 mg/L β-mercapto-ethanol (Gibco, catalog no. 21985–023), 1X HEPES (Gibco, catalog no. 15630–080), 1X penicillin/streptomycin (Gibco, catalog no. 15070–063), 1X sodium pyruvate (Gibco, catalog no. 11360–070), 1X MEM (Gibco, catalog no. 11140–050) and 1X l -glutamine (Gibco, catalog no. 25030–081). .. Activated PBMCs were incubated with XTX301, and proteolytically activated XTX301 using recombinant MMP14 (R&D systems, catalog no. 918-MPN) or rh-IL12 (R&D systems, catalog no. 219-IL-025/CF) at 37°C for 30 minutes. ..



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